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To determine if the V229A substitution affected receptor recruitment to clathrin-coated pits (CCPs) after agonist stimulation, TIRF images were acquired of both unstimulated and exendin-4-stimulated INS-1 832/3 SNAP/FLAG-hGLP-1R WT versus V229A cells (Figure 5C)

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The Swedish Obese Subjects study followed over 2,000 people receiving conventional weight-loss treatment at 480 Swedish primary care centres